Phospho eif2a

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August undefined, 2024

WebWestern Blot: eIF2A [p Ser51] Antibody [NB100-81896] - Analysis of extracts from Jurkat cells untreated or treated with H2O2 using eIF2 alpha (Phospho-Ser51) antibody. Immunocytochemistry/ Immunofluorescence: eIF2A [p Ser51] Antibody [NB100-81896] - Staining of methanol-fixed HeLa cells using eIF2a (Phospho-Ser51) antibody. WebTarget Information. Eukaryotic translation initiation factor 2 (EIF-2) functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA and binding to a 40S ribosomal subunit. EIF-2 is …

Initiation factor eIF2 alpha phosphorylation in stress

WebIn response to different environmental stresses, eIF2α phosphorylation represses global translation coincident with preferential translation of ATF4, a master regulator controlling the transcription of key genes essential for adaptative functions. WebRayBio® Phospho-eIF-2A (S52) and Total eIF-2A ELISA kit is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in human cell lysates. By determining phosphorylated eIF-2A protein in your experimental model system, you can verify pathway activation in your cell lysates. hideaways hotel exuma

eIF2α Antibody (D-3) SCBT - Santa Cruz Biotechnology

WebEIF2a (Eukaryotic translation initiation factor 2A) is a heterotrimer composed of three subunits (alpha, beta, and gamma). This translation initiation factor drives binding of … WebExtensive phosphorylation of eIF2 alpha and strong inhibition of eIF2B activity can result in the downregulation of the overall rate of protein synthesis; less marked changes may lead … WebJul 16, 2024 · eIF2α Antibody (D-3) is an IgG 1 κ mouse monoclonal eIF2 alpha antibody (also designated EIF2A antibody, eIF2 alpha antibody, or EIF2S1 antibody) that detects the eIF2 alpha protein of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA. eIF2α Antibody (D-3) is available as both the non-conjugated anti-eIF2 alpha antibody form, as … hideaways hotels südtirol

Phosphorylation of eukaryotic initiation factor-2α (eIF2α …

Phosphorylation of eIF2α at Serine 51 Is an Important Determinant …

WebPhospho-EIF2S1 (Ser49) Polyclonal Antibody, Invitrogen™-Rabbit Polyclonal Antibody Shop Phospho-EIF2S1 (Ser49) Polyclonal Antibody, Invitrogen™ at Fishersci.com Fisher Scientific ... EIF2a (Eukaryotic translation initiation factor 2A) is a heterotrimer composed of three subunits (alpha, beta, and gamma). This translation initiation factor ... WebThe PathScan ® Phospho-eIF2α (Ser51) Sandwich ELISA Kit #7286 detects endogenous levels of eIF2α protein when phosphorylated at Ser51 as shown in Figure 1. Kit sensitivity is shown in Figure 2. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species. howes loyalty rewardsWebTHUNDER™ Phospho-eIF2α (S51) + Total eIF2α TR-FRET Cell Signaling Assay Kit. Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-EIF2α (S51) and Total eIF2α in cell lysates. This unique kit allows to simultaneously detect phosphorylated and total eIF2α proteins from the same lysate sample in separate wells within the ... hideaways hotels gardasee

"WebEIF2a (Eukaryotic translation initiation factor 2A) is a heterotrimer composed of three subunits (alpha, beta, and gamma). This translation initiation factor drives binding of … " - Phospho eif2a

Phospho eif2a

The structural basis of translational control by eIF2 ... - Nature

WebHuman Phospho-eIF2 alpha (S52) Antibody Summary Species Reactivity Human Specificity Detects human Phospho-eIF2 alpha (S52) in ELISAs and Western blots. Source Monoclonal Rat IgG 2B Clone # 849159 Purification Protein A or G purified from hybridoma culture supernatant Immunogen Phosphopeptide containing the human eIF2 alpha S52 site WebOur eIF2A polyclonal, monoclonal and recombinant monoclonal antibodies are developed in Rabbit, Mouse and Goat. Find the eIF2A antibody that fits your needs. Choose from 1 of 14 eIF2A antibodies, which have been validated in experiments with 25 publications and 60 images featured in our data gallery.

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WebEIF2a (Eukaryotic translation initiation factor 2A) is a heterotrimer composed of three subunits (alpha, beta, and gamma). This translation initiation factor drives binding of initiator methionyl-tRNA to the 40S … WebHuman Phospho-eIF2 alpha (S52) Antibody Summary. Detects human Phospho-eIF2 alpha (S52) in ELISAs and Western blots. Lyophilized from a 0.2 μm filtered solution in PBS with …

WebFeb 26, 2015 · Phospho-eIF2α resistance has been observed both in yeast and in mammalian cells. In yeast, mutations in eIF2B (the GEF for eIF2) and eIF5 (the 48S PIC … WebAnti-EIF2S1 (phospho S51) antibody [E90] (ab32157) only detects eIF-2a phosphorylated on Serine 51. Tested applications Suitable for: WB, IHC-P, Dot blot more details Unsuitable for: Flow Cyt,ICC/IF or IP Species …

WebDescription. RNase H，即核糖核酸酶H，是一种核糖核酸内切酶，可特异性地水解DNA-RNA结合链中RNA磷酸二酯键。. RNase H具有双二价金属离子催化机制，如Mg2+和Mn2+直接参与催化功能。. RNase H不能水解单链或双链DNA或RNA中的磷酸二酯键。. 本试剂盒可用于cDNA第二链合成前 ... WebPhospho-eIF2alpha (Ser51) Antibody detects endogenous eIF2alpha only when phosphorylated at Ser51. The antibody does not recognize …

WebNov 2, 2024 · Eukaryotic initiation factor eIF2 is a key component of the ternary complex whose role is to deliver initiator tRNA into the ribosome. A variety of stimuli, both physiologic and pathophysiologic activate eIF2 kinases that phosphorylate the α subunit of eIF2, preventing it from forming the ternary complex, thus attenuating cellular protein …

WebJun 1, 2005 · Phospho-eIF2α restricts the activity of the guanine nuclear exchange factor eIF2B, which is responsible for the formation of the GTP–eIF2–tRNAi Met ternary complex. Because translation of most cellular and viral mRNA proceeds via ternary complex-dependent mechanisms, this leads to a global decrease in protein synthesis. howes logoWebThe ratio of phospho-eIF2α to total eIF2α was determined by scanning densitometry and image analysis (Image J, NCBI). ... It is possible that queuine reduces the phosphorylation level of eIF2a ... hideaway single bedWebThe phospho-EIF2 alpha assay is designed for the cell-based quantitative detection of Ser52 phosphorylation on EIF2 alpha. In NASH, lipid accumulation in hepatocytes (steatosis) … hideaways hunstantonWebPhosphorylation of the eukaryotic initiation factor 2 (eIF2) α subunit is a well-documented mechanism to downregulate protein synthesis under a variety of stress conditions. eIF2 binds GTP and Met-tRNAi and transfers Met-tRNA to the 40S subunit to form the 43S preinitiation complex (1,2). eIF2 promotes a new round of translation initiation by … howeslube save and winWebJun 23, 2024 · A mechanism based on reversible phosphorylation of the α-subunit of eukaryotic initiation factor 2 (eIF2α) has been confirmed as an important regulatory pathway for the inhibition of protein synthesis in mammalian and yeast cells, while plants constitute the significant exception. We studied the induction of TaeIF2α phosphorylation in … hideaways hotels portugalWeb需开启共用和该实验室部分空调净化系统，即可满足使用要求，不仅操控方便而且节约能源. 降低了运行成本。. 5 其他部分. 1)建筑围护方面. 设计选用金属夹芯作为实验室的壁、顶板，地面选用绿色环氧自流平无缝铺装，板材之. 间及板材与地面相交处选用R50的 ... howes loyalty treatsWebEIF2a (Eukaryotic translation initiation factor 2A) is a heterotrimer composed of three subunits (alpha, beta, and gamma). This translation initiation factor drives binding of initiator methionyl-tRNA to the 40S ribosome in an AUG-dependent manner to form the 43S pre-initiation complex. hideaways in history winter promise