Transcriptional repressor CTCF also known as 11-zinc finger protein or CCCTC-binding factor is a transcription factor that in humans is encoded by the CTCF gene. CTCF is involved in many cellular processes, including transcriptional regulation, insulator activity, V(D)J recombination and regulation … See more CCCTC-Binding factor or CTCF was initially discovered as a negative regulator of the chicken c-myc gene. This protein was found to be binding to three regularly spaced repeats of the core sequence CCCTC and thus … See more The primary role of CTCF is thought to be in regulating the 3D structure of chromatin. CTCF binds together strands of DNA, thus forming chromatin loops, and anchors DNA to cellular structures like the nuclear lamina. It also defines the boundaries between active and … See more CTCF binds to itself to form homodimers. CTCF has also been shown to interact with Y box binding protein 1. CTCF also co-localizes with cohesin, which extrudes chromatin loops by … See more • CCCTC-binding+factor at the U.S. National Library of Medicine Medical Subject Headings (MeSH) • FactorBook CTCF See more The binding of CTCF has been shown to have many effects, which are enumerated below. In each case, it is unknown if CTCF directly evokes the … See more CTCF binds to the consensus sequence CCGCGNGGNGGCAG (in IUPAC notation). This sequence is defined by 11 zinc finger motifs … See more • Ohlsson R, Renkawitz R, Lobanenkov V (2001). "CTCF is a uniquely versatile transcription regulator linked to epigenetics and … See more WebPD-L1 enhances the CSC-like properties of GC cells via binding CTCF. (A) The expression of CD44, Nanog, Oct4, and N-cadherin in SGC-7901 following siRNA mediated knockdown of CTCF and GCMSC-CM treatment for 24 h were detected by western blot.
CTCF - an overview ScienceDirect Topics
WebFeb 23, 2024 · To investigate the roles of CTCF in chromatin architecture and transcription, we fused AID and mCherry proteins at the C terminus of endogenous CTCF in an … WebFeb 10, 2024 · The ~10–15% Hoxa7-P2A-eGFP-positive cells (Extended Data Fig. 1c,d) allowed for enough of a dynamic range to identify mutants that decreased or increased CTCF insulating properties. grand turk hospital
DNA architectural protein CTCF facilitates subset-specific …
WebAug 12, 2024 · This mathematical property of the CTCF grammar led us to hypothesise that divergent and convergent CTCF sites occur at different spatial regimes and that this underlies the emerging dimensions of TADs and their boundaries. We therefore analysed the distributions of CTCF cluster sizes along the human genome. This revealed that, at … WebCTCF is a highly conserved, multifunctional zinc finger protein involved in critical aspects of gene regulation including transcription regulation, chromatin insulation, genomic imprinting, X-chromosome inactivation, and higher order chromatin organization. Such multifunctional properties of CTCF suggest an essential role in development. WebFeb 3, 2024 · Most domain boundaries in mouse and human cells are occupied by the CCCTC-binding factor (CTCF), which binds to motifs in a convergent orientation at the two boundaries (13, 14).Mechanistically, TADs are thought to form by a loop-extrusion mechanism in vertebrates, whereby a chromatin loop is extruded by the cohesin complex … grand turk island car rental