Western blot analysis of extracts from HeLa cells (lane 1) or SMAD2 knock-out cells (lane 2) using Smad2 (86F7) Rabbit mAb #3122 (upper), and α-Actinin (D6F6) XP ® Rabbit mAb #6487 (lower). The absence of signal in the SMAD2 knock-out HeLa cells confirms specificity of the antibody for SMAD2. WebJul 23, 2016 · Clones Smad2,Smad3 Smad7siRNA least90% inhibition targetgenes were chosen furtheranalysis. experimentalprocedures, second thirdpassages eachclone were used. knockdowncells monitoredduring culture under invertedmicroscope. ... were performed codedslides blindedfashion. Western blot Low-molecular weight marker (Cell Signaling …
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WebJan 17, 2024 · Additionally, JQ1 suppressed BRD4, c-MYC, Collagen I, TGF-β, p-NF-κB p65, p-Smad2 and p-Smad3 expressions after irradiation, repressed proliferation and transdifferentiation of lung fibroblasts ... WebSep 27, 2024 · The antibodies used for ChIP-qPCR were anti-SMAD3 (CST and C67H9), anti-SMAD4 (CST and D3R4N), and anti-USP7(Bethyl and A300-033A). All ChIP-qPCR primers are listed in Supplementary Table S1 . RNA ... shannon sparks facebook
Levels of phospho-Smad2/3 are sensors of the interplay between …
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