Cst atf4 antibody

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August undefined, 2024

WebAug 13, 2024 · This was especially relevant as we found in the public CHIP-seq data base (chip-atlas.org), that the Nrf2 antibody bound to the ATF4 promoter (~1 kB from the TSS) in the cardiovascular HAEC cell ... WebMonoclonal Antibody for studying ATF-4. Cited in 661 publications. Validated for WB, IP, IF, ChIP, ChIP, C&R. Highly specific and rigorously …

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WebMar 25, 2024 · Western Blot analysis of Rat Brain showing detection of ~39 kDa (isoform 2) ATF4 protein using Mouse Anti-ATF4 Monoclonal Antibody, Clone S360A-24 (56583). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Brain. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-ATF4 Monoclonal Antibody (56583) at … WebMar 1, 2024 · The SLC7A11 antibody is validated for use in MEFs in Figure 6—figure supplement 1A, corresponding immunoblots quantified in Figure 6—figure supplement 1B, C, F. ... ATF4 rabbit monoclonal: Cell … how many presidents were there before george

Activating mutations in EGFR and PI3K promote ATF4 induction …

WebDec 17, 2024 · BackgroundThe role of activating transcription factor 4 (ATF4) underlying gastric cancer (GC) remains unclear. The purpose of this study was to investigate the expression levels and biological functions of ATF4 in GC.MethodsExpression of ATF4 was detected by quantitative PCR (qPCR), Western blotting, and immunohistochemistry. Cox … WebDec 25, 2024 · The membrane was blocked and then incubated overnight at 4°C with primary antibody as described previously ... antibody to phospho-T389 p70S6K1 was obtained from Cell Signaling Technology (catalog number: 9205); antibodies to REDD1 (catalog number: 10638-1-AP), Sestrin1 (catalog number: 21668-1-AP), and Sestrin2 … Web1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human ATF4 (60035-1-Ig, Clone:3E4C5) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG (H+L) at dilution 1:1000 (red), or 0.4 ug Control … how cook pork chop

Anti-ATF-4 (phospho S245) antibody (ab28830) Abcam

Hepatic mTORC1 signaling activates ATF4 as part of its …

Webab31390 has been referenced in 9 publications. Liu Y Hydrogen peroxide induces nucleus pulposus cell apoptosis by ATF4/CHOP signaling pathway. Exp Ther Med 20:3244-3252 (2024). PubMed: 32855694; Liu P et al. … WebThis antibody has been shown to work in applications such as: Precipitation, Sequencing, ChIP-Seq, ChIP, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Western Blot, and Other. ATF-4 (D4B8) Rabbit … how cook potatoes in ovenWebMar 25, 2024 · Some oncoproteins along with stress kinase general control non-derepressible 2 (GCN2) can ensure the induction of activating transcription factor 4 (ATF4) to counteract amino acid deprivation; however, little is known regarding the role of the oncogenic EGFR-PI3K pathway. In this study, we demonstrate that both mutated EGFR … how many presidents were there before lincoln

"WebJun 24, 2024 · PERK IPs were performed with 1 mg of lysate incubated with 2 µg of primary antibody (Proteintech, #20582-1AP) or the corresponding IgG (Cell Signaling Technology, #2729) overnight at 4 °C with ... " - Cst atf4 antibody

Cst atf4 antibody

ATF-4 (D4B8) Rabbit mAb Cell Signaling Technology

WebMay 5, 2024 · About 500 mg protein of total cell lysates was incubated with primary antibody anti-ATF4 (#11815, CST or 10835-1-ap, Proteintech group, USA) or anti-CTCF (#3418, CST or sc271514, Santa Cruz Biotechnology, USA) at 4 °C with overnight shaking. The immune complexes were then incubated with Protein A/G Dynabeads (Thermo … WebJun 14, 2013 · The transcription factor activating transcription factor 4 (ATF4) belongs to the cAMP-responsive element–binding protein family, characterized by the presence of a leucine zipper dimerization domain and a basic amino acid–rich DNA binding domain ().Previous studies have shown that knocking out ATF4 gene expression in mice results …

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WebThe antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these … WebMonoclonal Antibody for studying ATF-4. Cited in 3 publications. Validated for Western Blotting, Immunofluorescence (Immunocytochemistry). Highly specific and rigorously …

WebFeb 17, 2024 · Autophagy is a cellular process through which cells engulf and degrade damaged cytoplasmic components ().Recent studies have implicated the involvement of autophagy in the regulation of obesity and energy expenditure (15–21).For example, autophagy is reduced in the hypothalamus of aged mice or mice fed an HFD … WebNov 1, 2024 · Membranes were then incubated at 4 °C overnight with ATF4 antibody (BioLegend) at 1:1000. After washing with 1x TBST, anti-rat HRP secondary antibody (CST) was used at 1:2000. A list of commercially available antibodies tested in livers and cell lines is included in Supplementary Table 1. 2.6. Gene expression analysis.

Web(11815) ATF-4 (D4B8) Rabbit mAb - Cell Signaling Technology - CiteAb. 670 citations – A rabbit monoclonal antibody, raised against Cyclic AMP-dependent transcription factor ATF-4 (Human), supplied by Cell Signaling Technology. ... ATF4, Activating transcription factor 4, CREB-2, CREB2, Cyclic AMP-dependent transcription factor ATF-4, Cyclic ... WebSep 29, 2024 · ATF-4 Antibody (B-3) is an IgG 2b κ mouse monoclonal ATF-4 antibody (also designated ATF4 antibody, cAMP-dependent transcription factor ATF-4 antibody, CREB-2 antibody or cAMP …

WebOct 22, 2010 · ATF4 is a transcriptional activator of the integrated stress response, a program of gene expression involved in metabolism, nutrient uptake, anti-oxidation, and the activation of additional transcription factors, such as CHOP/GADD153, that can induce apoptosis. ... (Cell Signaling Technologies). ATF4 antibody was prepared against …

WebThis antibody is a rabbit polyclonal antibody raised against full length human ATF4 antigen. The antibody recognizes the 38 kDa ATF4 protein and its phosphorylated … how many presidents were there in the 1980sWebImmunoprecipitation of ATF-4 from extracts of 293 cells, treated with tunicamycin (2 μg/ml, 8 hr), using Rabbit (DA1E) mAb IgG XP ® Isotype … how cook polish sausageWebCST4. Cystatin-S is a protein that in humans is encoded by the CST4 gene. [3] [4] The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. … how many presidents were there in indiaWebATF4 Antibody - Mouse monoclonal antibody to human Activating Transcription Factor 4, clone S360A-24. Validated for WB, IHC, ICC/IF with human, rat tissue. ... Cell Signaling, Epigenetics and Nuclear Signaling, Neuroscience : Cellular Localization : Cell membrane, Cytoplasm, Nucleus : Accession Number : NP_001666.2 ... how cook quailWebAnti-ATF4 antibodies are offered by a number of suppliers. This target gene encodes the protein 'activating transcription factor 4' in humans and may also be known as CREB-2, … how cook pot roast in crock potWebHowever, CS-11815 detects a band with a mass of ~49 kDa, while the expected molecular weight of ATF4 is ~38 kDa. In contrast, SC-200 is a very dirty polyclonal antibody, but it seems to detect ... how cook polentaWebDec 19, 2024 · Shipping Temperature : Blue Ice or 4ºC. Cite this Product : StressMarq Biosciences Cat# SMC-447D, RRID: AB_2701793. Certificate of Analysis : 1 µg/ml of SMC-447 was sufficient for detection of ATF4 in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. how cook pot roast